Cardiac Histopathological and Immunohistochemichal Changes due to Electrical Injury in Rats

Document Type : Original Article

Authors

1 Department of Forensic Medicine and Clinical Toxicology; Faculty of Medicine, Assiut University, Egypt

2 Department of Pathology, Faculty of Medicine, Assiut University, Egypt

3 Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Assiut University, Egypt

Abstract

The aim of this study was to detect the histopathological findings in the myocardium after electrical injury in rats and the potential role of c- fos expression as a marker to distinguish between antemortem and postmortem electrocution. Seventy healthy female rats were included in the study and randomly divided into four groups (A,B,C,D). Group (A): Twenty rats were subjected to instantaneous antemortem electricity, and were divided randomly into two subgroups. Group (A1): Ten rats were subjected to cervical dislocation and the hearts were collected immediately. Group (A2): Ten rats were left alive for1h from electrical injury then hearts were collected after cervical dislocation. Group (B): Twenty rats were electrically injured instantaneously postmortem, after death by cervical dislocation, and were also divided into two subgroups. Group (B1):  Hearts were collected immediately in 10 rats. Group (B2): Hearts were collected after 1h from electrical injury in the other 10 rats.  Group (C): Twenty rats were electrified up to death, and divided randomly into two subgroups. Group (C1):  Hearts of 10 rats were collected immediately. Group (C2):  Hearts of the other 10 rats were collected after 1h of death from electricity. Lastly, 10 rats were served as the control group (Group D). Group (D1): Five rats were clamped but not electrified, before death by cervical dislocation. Group (D2): other 5 rats were clamped but not electrified, after being killed by cervical dislocation. Sections from the hearts of all groups were fixed in formalin and routinely processed. c- fos expression was evaluated in all groups by immunohistochemistry. Significant histopathological findings were detected in groups A and C. Few c-fos oncogene protein positive cardiomyocyte nuclei were seen in rats of groups (A1) and (B1). Positive expression of c-fos protein increased in rats of groups C1, C2 and A2. No c-fos oncogene protein expression was seen either in control groups or in group B2. Significant differences in c-fos oncogene protein expression were observed between rats of groups A1, A2, C1 and C2. Thus, c-fos can be regarded as a marker in identifying electrical injury, and can be used as an indicator to distinguish between antemortem and postmortem electrocution. 

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